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10-Methoxycamptothecin

10-Methoxycamptothecin

Catalog No. BCN2303
Size Price Stock
20mg $138 In stock
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Quality Control of 10-Methoxycamptothecin

Chemical structure

10-Methoxycamptothecin

Biological Activity of 10-Methoxycamptothecin

1. 10-Methoxycamptothecin possesses high anti-cancer activity.

10-Methoxycamptothecin Dilution Calculator

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Chemical Properties of 10-Methoxycamptothecin

Cas No. 19685-10-0 SDF Download SDF
SMILES CC[C@@]1(O)C(=O)OCC2=C1C=C3N(Cc4cc5cc(OC)ccc5nc34)C2=O
Standard InChIKey KLFJSYOEEYWQMR-NRFANRHFSA-N
Standard InChI InChI=1S/C21H18N2O5/c1-3-21(26)15-8-17-18-12(6-11-7-13(27-2)4-5-16(11)22-18)9-23(17)19(24)14(15)10-28-20(21)25/h4-8,26H,3,9-10H2,1-2H3/t21-/m0/s1
Type of Compound Alkaloids Appearance Cryst.
Formula C21H18N2O5 M.Wt 378.38
Solubility Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
Shipping Condition Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other courier with RT , or blue ice upon request.

Preparing Stock Solutions of 10-Methoxycamptothecin

1 mg 5 mg 10 mg 20 mg 25 mg
1 mM 2.6428 mL 13.2142 mL 26.4285 mL 52.8569 mL 66.0711 mL
5 mM 0.5286 mL 2.6428 mL 5.2857 mL 10.5714 mL 13.2142 mL
10 mM 0.2643 mL 1.3214 mL 2.6428 mL 5.2857 mL 6.6071 mL
50 mM 0.0529 mL 0.2643 mL 0.5286 mL 1.0571 mL 1.3214 mL
100 mM 0.0264 mL 0.1321 mL 0.2643 mL 0.5286 mL 0.6607 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.

Preparation of 10-Methoxycamptothecin

This product is isolated and purified from the barks of Camptotheca acuminata Decne

References on 10-Methoxycamptothecin

A simple HPLC method with fluorescence detection for simultaneous determination of 10-methoxycamptothecin and its metabolite 10-hydroxycamptothecin in rat liver tissue.[Pubmed: 24782285]


A simple HPLC method to determine the amount of 10-Methoxycamptothecin (MCPT) and its major metabolite 10-hydroxycamptothecin (HCPT) in rat liver tissue was developed in the present study. Camptothecin (CPT) was used as internal standard (IS). A piecewise linear function was used over lower and higher concentrations, respectively. The calibration curves were linear (r (2) >0.99) over concentrations from 2.5 to 20 ng/mL and 20 to 320 ng/mL for both MCPT and HCPT. The method had an accuracy of 92.74% to 112.76%, and the intra- and inter-day precision (RSD%) were 11.85% or less for MCPT and HCPT. The stability data showed no significant degradation occurred under the experimental conditions. This method was successfully applied to the tissue distribution study of MCPT and its metabolite HCPT in liver tissue samples after intravenous administration.

Development and validation of a RP-HPLC method with fluorescence detection for simultaneous determination of 10-methoxycamptothecin and its metabolite 10-hydroxycamptothecin in rat plasma.[Pubmed: 22824730]


Both 10-Methoxycamptothecin (MCPT) and 10-hydroxycamptothecin (HCPT) are the natural bioactive derivatives of camptothecin (CPT) isolated from Camptotheca acuminata, and have been confirmed to possess high anti-cancer properties. In the present study, HCPT was identified as the major metabolite of 10-Methoxycamptothecin in rat plasma through HPLC/photodiode array detection (PDA) and LC-MS/MS analysis. A sensitive and reliable RP-HPLC method with fluorescence detection was developed and validated for the simultaneous analysis of 10-Methoxycamptothecin and HCPT in rat plasma. The parental CPT was used as an internal standard (IS). A piecewise linear function was used over lower and higher concentrations, respectively. The calibration curves were linear (r² > 0.999) over concentrations from 1.25 to 20 ng/mL and 20 to 320 ng/mL for both 10-Methoxycamptothecin and HCPT. The method had an accuracy of 92.24-113.90%, and the intra- and inter-day precision (RSD%) were 10.05% or less for 10-Methoxycamptothecin and HCPT. The stability data showed no significant degradation occurred under the experimental conditions. The mean recoveries at concentrations of 2.5, 40 and 160 ng/mL were 95.09±3.94%, 98.67±1.40% and 95.65±2.15% for 10-Methoxycamptothecin and 84.06±4.39%, 84.85±3.10% and 81.03±4.44% for HCPT, respectively. The lower limit of quantification (LLOQ) using 0.1 mL of plasma was 1.25 ng/mL for both MCPT and HCPT. This method was successfully applied to the pharmacokinetic study of 10-Methoxycamptothecin and its metabolite HCPT in rat plasma after intravenous administration.

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