Curculigo orchioides (commonly called Golden Eye-grass or Xian Mao) is a flowering plant species in the genus Curculigo. In C. orchidoides, curculigoside A, B, C and D and curculigine A and D can be found. Curculigosides are natural phenols found in C. orchioides that could be useful against β-amyloid aggregation in Alzheimer’s disease. Three new phenolic glycosides, curculigosides F–H (1–3), were isolated from rhizomes of Curculigoorchioides Gaertn. Their structures were elucidated based on comprehensive spectroscopic analyses including IR, MS, 1D- and 2D NMR (HSQC, COSY, and HMBC). Curculigosides F–H (1–3) were evaluated for their anti-HBV activity in vitro using the HBV transfected Hep G2.2.15 cell line. Sustained hyperglycemia is considered as a major cause of sexual and erectile dysfunction in human population. Curculigo orchioides (CO) is considered as a sexual tonic in Ayurvedic system of medicine with potent antioxidant and adaptogenic properties.