Cistanche deserticola is a holoparasitic member of the Orobanchaceae family of plants, commonly known as desert-broomrape. The plant lacks chlorophyll and obtains its nutrients and water in a parasitic fashion from the black saxaul (Haloxylon ammodendron) and white saxaul (Haloxylon persicum). Five phenylethanoid glycosides (PhGs), echinacoside, cistanoside A, acteoside, isoacteoside and 2′-acetylacteoside, were isolated and purified from Cistanchedeserticola for the first time by high-speed counter-current chromatography (HSCCC) using two biphasic systems, one consisting of ethyl acetate–ethanol–water (5:0.5:4.5, v/v/v) and another of ethyl acetate–n-butanol–ethanol–water (0.5:0.5:0.1:1, v/v/v/v). A total of 28.5 mg of echinacoside, 18.4 mg of cistanoside A, 14.6 mg of acteoside, 30.1 mg of isoacteoside and 25.2 mg of 2′-acetylacteoside were purified from 1412 mg of the n-butanol extract of C. deserticola, each at over 92.5% purity as determined by HPLC.