Methylophiopogonanone A

Methylophiopogonanone A Protects against Cerebral Ischemia/Reperfusion Injury and Attenuates Blood-Brain Barrier Disruption In Vitro.[Pubmed: 25897666]

PLoS One. 2015 Apr 21;10(4):e0124558.

Methylophiopogonanone A (MO-A), an active homoisoflavonoid of the Chinese herb Ophiopogon japonicus which has been shown to have protective effects on cerebral ischemia/reperfusion (I/R) injury, has been demonstrated to have anti-inflammatory and anti-oxidative properties. However, little is known about its role in cerebral I/R injury.
METHODS AND RESULTS:
Therefore, in this study, by using a middle cerebral artery occlusion (MCAO) and reperfusion rat model, the effect of MO-A on cerebral I/R injury was examined. The results showed that MO-A treatment reduced infarct volume and brain edema, improved neurological deficit scores, reversed animal body weight decreases, and increased animal survival time in the stroke groups. Western blotting showed that MO-A suppressed MMP-9, but restored the expression of claudin-3 and claudin-5. Furthermore, transmission electron microscopy were monitored to determine the blood-brain barrier (BBB) alterations in vitro. The results showed that MO-A markedly attenuated BBB damage in vitro. Additionally, MO-A inhibited ROS production in ECs and MMP-9 release in differentiated THP-1 cells in vitro, and suppressed ICAM-1 and VCAM-1 expression in ECs and leukocyte/EC adhesion.
CONCLUSIONS:
In conclusion, our data indicate that MO-A has therapeutic potential against cerebral I/R injury through its ability to attenuate BBB disruption by regulating the expression of MMP-9 and tight junction proteins.

Chinese Traditional & Herbal Drugs, 2015, 46(20):3091-5.

Comparison on homoisoflavones extracted from Ophiopogon japonicus in Sichuan and Zhejiang and their anti-oxidative activity.[Reference: WebLink]

To compare the contents of three homoisoflavones and their anti-oxidative activity of Ophiopogon japonicus in Sichuan(OJS) and Zhejiang(OJZ).
METHODS AND RESULTS:
The determination was performed on ACQUITYTM UPLC BEH C18 column(100 mm × 2.1 mm, 1.7 μm), the Ultra Performance Liquid Chromatography-Photo-Diode Array(UPLC-PDA) technology was applied, and the mobile phase consisted of acetonitrile and 0.2% formic acid aqueous solution(55 : 45) with gradient elution program, flow rate was 0.20 m L/min with 2 μL of sample quantity at 296 nm, and the anti-DPPH radical efficiency of water extract from Ophiopogonis Radix were evaluated by UV-photometer. The average values of methylophiopogonone A, Methylophiopogonanone A, and methylophiopogonanone B were(3.06 ± 0.54),(40.10 ± 5.63), and(29.51 ± 5.06) μg/g in OJS, respectively; while those in OJZ were(9.22 ± 3.52),(106.63 ± 27.56), and(256.97 ± 61.79) μg/g, separately. The IC50 value was 16.59 mg/m L in OJS, while that in OJZ was 14.48 mg/m L. The IC50 value of positive control VC was 7.06 μg/m L.
CONCLUSIONS:
Compared with OJS, the contents of homoisoflavones in OJZ are higher, and the anti-radical efficiency of water extract from OJZ is stronger. It provides the basis for the quality evaluation and geo-authentic research of Ophiopogonis Radix.