Flurofamide

The failure of flurofamide to control ureaplasma in bovine semen.[Pubmed:3756683]

Can J Vet Res. 1986 Apr;50(2):289-90.

The Flurofamide sensitivities of 21 bovine ureaplasma isolates were determined using the metabolic inhibition method. The 21 isolates included seven each of vaginal, preputial and seminal origin. The minimum inhibitory concentrations of Flurofamide ranged from 0.0125 to 0.2 meg/mL against a ureaplasma titer of 10(4) organisms/mL. The minimum lethal concentrations ranged from less than or equal to 0.1 to 3.2 mcg/mL. Flurofamide was then evaluated in a system comparable to the procedure for semen extension with respect to temperature, time and dilution. The compound was found to be ineffective in reducing ureaplasma numbers in this system at levels up to 1500 mcg/mL.

Ureaplasma-urealyticum-induced bladder stones in rats and their prevention by flurofamide and doxycycline.[Pubmed:3667226]

Isr J Med Sci. 1987 Jun;23(6):565-7.

Struvite calculi can be produced in the bladder of Sprague-Dawley male rats after injection of ureaplasmas into the renal medulla. Calculi appear 3 to 6 days after ureaplasma injection. We have studied the inhibitory effect of Flurofamide, a potent inhibitor of Ureaplasma urealyticum urease, and doxycycline, on the formation of bladder stones. Flurofamide given orally in five doses (total 125 mg) over 3 days and doxycycline in seven doses (total 20 mg) over 4 days partially prevented stone formation only when given at the time of inoculation. Ureaplasmas disappeared rapidly from the urine. The inhibitory effect of Flurofamide was higher than that of doxycycline. However, doxycycline seemed to be efficient when given for a long period (5 weeks).

Marked reduction of Helicobacter pylori-induced gastritis by urease inhibitors, acetohydroxamic acid and flurofamide, in Mongolian gerbils.[Pubmed:11453654]

Biochem Biophys Res Commun. 2001 Jul 20;285(3):728-33.

Urease has been suggested to be essential for colonization and pathogenesis of Helicobacter pylori infection. In the present study, we evaluated the effects of urease inhibitors [acetohydroxamic acid (AHA) and Flurofamide (FFA)] on H. pylori-induced gastritis in Mongolian gerbils. Animals were orally inoculated with H. pylori, and given urease inhibitors in their diet throughout the experimental period of six weeks or four weeks, starting from two weeks after H. pylori inoculation. With the administration of AHA at doses of 100, 500, and 2500 ppm throughout the experimental period, H. pylori-induced gastritis in animals was decreased in a dose-dependent manner, significantly so at 2500 ppm. Suppression of gastric lesions was also evident in animals administered 2500 ppm AHA after the H. pylori infection. Bacterial infection rates were reduced to 40-50% of the control value of 100%, by the highest dose of AHA. The potent urease inhibitor, FFA, also caused marked amelioration of H. pylori-associated gastritis on administration at 100 ppm throughout the six-week experimental period or for four weeks after H. pylori infection. Animals treated with FFA had few visible gastric lesions, and the proportion infected with H. pylori was reduced to less than 10%. Since antibiotic-resistant strains of H. pylori have become a serious problem, nonantibiotic urease inhibitors may be very useful to control H. pylori-associated gastroduodenal disease.

Investigation into the inhibitory effect of flurofamide on animal ureaplasmas and its use in the treatment of ureaplasma-infected sheep.[Pubmed:3761418]

J Vet Pharmacol Ther. 1986 Sep;9(3):280-5.

In vitro tests with the urease inhibitor Flurofamide demonstrated that the final inhibitory concentration of 0.5-4 microM on the growth of nine ureaplasma strains was largely ureaplasmastatic, requiring prolonged incubation to have a ureaplasmacidal effect. Intramuscular injection of Flurofamide successfully eliminated genital infections of ureaplasma in sheep only when the treatment was repeated on two consecutive days. A dose rate of 5-20 mg/kg body weight eliminated the organism from naturally infected sheep, but 15-25 mg/kg body weight was required to eliminate the infection from eleven of fourteen experimentally, newly infected sheep. Administration of the Flurofamide orally in the drinking water failed to eliminate ureaplasmas from any of twenty newly infected sheep.