3-epi-BufalinCAS# 465-20-3 |
Quality Control & MSDS
Number of papers citing our products
Chemical structure
| Cas No. | 465-20-3 | SDF | Download SDF |
| PubChem ID | N/A | Appearance | Powder |
| Formula | C24H34O4 | M.Wt | 386.53 |
| Type of Compound | Steroids | Storage | Desiccate at -20°C |
| Solubility | Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc. | ||
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months. Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it. |
||
| About Packaging | 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial. 2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial. 3. Try to avoid loss or contamination during the experiment. |
||
| Shipping Condition | Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request. | ||
3-epi-Bufalin Dilution Calculator
3-epi-Bufalin Molarity Calculator
| 1 mg | 5 mg | 10 mg | 20 mg | 25 mg | |
| 1 mM | 2.5871 mL | 12.9356 mL | 25.8712 mL | 51.7424 mL | 64.678 mL |
| 5 mM | 0.5174 mL | 2.5871 mL | 5.1742 mL | 10.3485 mL | 12.9356 mL |
| 10 mM | 0.2587 mL | 1.2936 mL | 2.5871 mL | 5.1742 mL | 6.4678 mL |
| 50 mM | 0.0517 mL | 0.2587 mL | 0.5174 mL | 1.0348 mL | 1.2936 mL |
| 100 mM | 0.0259 mL | 0.1294 mL | 0.2587 mL | 0.5174 mL | 0.6468 mL |
| * Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations. | |||||
Calcutta University
University of Minnesota
University of Maryland School of Medicine
University of Illinois at Chicago
The Ohio State University
University of Zurich
Harvard University
Colorado State University
Auburn University
Yale University
Worcester Polytechnic Institute
Washington State University
Stanford University
University of Leipzig
Universidade da Beira Interior
The Institute of Cancer Research
Heidelberg University
University of Amsterdam
University of Auckland
TsingHua University
The University of Michigan
Miami University
DRURY University
Jilin University
Fudan University
Wuhan University
Sun Yat-sen University
Universite de Paris
Deemed University
Auckland University
The University of Tokyo
Korea University
- Araloside C
Catalog No.:BCX1266
CAS No.:55446-15-6
- Dihydrolanosterol
Catalog No.:BCX1265
CAS No.:911660-54-3
- Tarasaponin IV
Catalog No.:BCX1264
CAS No.:156980-31-3
- Fallacinol
Catalog No.:BCX1263
CAS No.:569-05-1
- Isoasiaticoside
Catalog No.:BCX1262
CAS No.:948827-09-6
- Tenacissoside A
Catalog No.:BCX1261
CAS No.:107352-30-7
- cis-Pellitorine
Catalog No.:BCX1260
CAS No.:639086-18-3
- Brevicornin
Catalog No.:BCX1259
CAS No.:173792-49-9
- Stipuleanoside R2
Catalog No.:BCX1258
CAS No.:96627-72-4
- Tibesaikosaponin V
Catalog No.:BCX1257
CAS No.:2319668-87-4
- Tenaxin I
Catalog No.:BCX1256
CAS No.:86926-52-5
- Ophiopogonanone D
Catalog No.:BCX1255
CAS No.:1027912-99-7
- Scheffoleoside A
Catalog No.:BCX1268
CAS No.:160669-23-8
- Demethyldaphnoretin-7-O-glucoside
Catalog No.:BCX1269
CAS No.:438578-91-7
- Protoanemonin
Catalog No.:BCX1270
CAS No.:108-28-1
- Sinomenine N-oxide
Catalog No.:BCX1271
CAS No.:1000026-77-6
- 6-Hydroxyluteolin
Catalog No.:BCX1272
CAS No.:18003-33-3
- Phenoxodiol
Catalog No.:BCX1273
CAS No.:81267-65-4
- 2'-O-Methylphloretin
Catalog No.:BCX1274
CAS No.:111316-17-7
- 2',4,4',6'-Tetramethoxychalcone
Catalog No.:BCX1275
CAS No.:94103-36-3
- Hispidol
Catalog No.:BCX1276
CAS No.:5786-54-9
- N-Acetylcytisine
Catalog No.:BCX1277
CAS No.:6018-52-6
- Palvanil
Catalog No.:BCX1278
CAS No.:69693-13-6
- Micromarin F
Catalog No.:BCX1279
CAS No.:73292-93-0
Simultaneous Determination of Bufalin and Its Nine Metabolites in Rat Plasma for Characterization of Metabolic Profiles and Pharmacokinetic Study by LC(-)MS/MS.[Pubmed:31035327]
Molecules. 2019 Apr 28;24(9):1662.
Characterization and determination of metabolites to monitor metabolic pathways play a paramount role in evaluating the efficacy and safety of medicines. However, the separation and quantification of metabolites are rather difficult due to their limited contents in vivo, especially in the case of Chinese medicine, due to its complexity. In this study, an effective and convenient method was developed to simultaneously quantify bufalin and its nine metabolites (semi-quantitation) in rat plasma after an oral administration of 10 mg/kg to rats. The prototype and metabolites that were identified were subsequently quantified using positive electrospray ionization in multiple reaction monitoring (MRM) mode with transitions of m/z 387.4-->369.6 and 387.4-->351.3 for bufalin, m/z 513.7-->145.3 for IS, and 387.4-->369.6, 419.2-->365.2, and 403.2-->349.2 for the main metabolites (3-epi-Bufalin, dihydroxylated bufalin, and hydroxylated bufalin, respectively). The method was validated over the calibration curve range of 1.00-100 ng/mL with a limit of quantitation (LOQ) of 1 ng/mL for bufalin. No obvious matrix effect was observed, and the intra- and inter-day precisions, as well as accuracy, were all within the acceptable criteria in this method. Then, this method was successfully applied in metabolic profiling and a pharmacokinetic study of bufalin after an oral administration of 10 mg/kg to rats. The method of simultaneous determination of bufalin and its nine metabolites in rat plasma could be useful for pharmacokinetic-pharmacodynamic relationship research of bufalin, providing experimental evidence for explaining the occurrence of some adverse effects of Venenum Bufonis and its related preparations.
Microbial transformation of resibufogenin by Curvularia lunata AS 3.4381.[Pubmed:24456251]
J Asian Nat Prod Res. 2014;16(3):290-5.
In this paper, the microbial transformation of resibufogenin by Curvularia lunata AS 3.4381 was investigated, and four transformed products were isolated and characterized as 3-epi-resibufogenin (2), 12alpha-hydroxy-3-epi-resibufogenin (3), 12-oxo-16beta-hydroxy-3-epi-resibufogenin (4), and 12beta,15-epoxy-3-epi-Bufalin-14,15-ene (5). Among them, 4 and 5 are new compounds, and isomerization, hydroxylation, and oxidation reactions in microbial transformation process were observed. Additionally, the cytotoxicities of transformed products (2-5) were also investigated.
