PseudocolumbamineCAS# 64191-04-4 |
Quality Control & MSDS
Number of papers citing our products
Chemical structure
| Cas No. | 64191-04-4 | SDF | Download SDF |
| PubChem ID | N/A | Appearance | Yellow powder |
| Formula | C20H20NO4 | M.Wt | 338.4 |
| Type of Compound | Alkaloids | Storage | Desiccate at -20°C |
| Solubility | Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc. | ||
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months. Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it. |
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| About Packaging | 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial. 2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial. 3. Try to avoid loss or contamination during the experiment. |
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| Shipping Condition | Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request. | ||
Pseudocolumbamine Dilution Calculator
Pseudocolumbamine Molarity Calculator
| 1 mg | 5 mg | 10 mg | 20 mg | 25 mg | |
| 1 mM | 2.9551 mL | 14.7754 mL | 29.5508 mL | 59.1017 mL | 73.8771 mL |
| 5 mM | 0.591 mL | 2.9551 mL | 5.9102 mL | 11.8203 mL | 14.7754 mL |
| 10 mM | 0.2955 mL | 1.4775 mL | 2.9551 mL | 5.9102 mL | 7.3877 mL |
| 50 mM | 0.0591 mL | 0.2955 mL | 0.591 mL | 1.182 mL | 1.4775 mL |
| 100 mM | 0.0296 mL | 0.1478 mL | 0.2955 mL | 0.591 mL | 0.7388 mL |
| * Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations. | |||||
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Characterization of Acetylcholinesterase Inhibitory Constituents from Annona glabra Assisted by HPLC Microfractionation.[Pubmed:20828184]
J Nat Prod. 2010 Oct 22;73(10):1632-5.
The active fraction of the EtOH extract of the stem of Annona glabra against acetylcholinesterase (AChE) was analyzed by combining HPLC microfractionation with a bioassay. The analytical-scale sample was fractionated by HPLC-DAD into 96-well microplates, which, after evaporation, were assayed against AChE. The active subfractions were scaled up by separation over semipreparative HPLC to give 20 compounds. Four of these, (7S,14S)-(-)-N-methyl-10-O-demethylxylopinine salt (3), S-(-)-7,8-didehydro-10-O-demethylxylopininium salt (10), S-(-)-7,8-didehydrocorydalminium salt (11), and 5-O-methylmarcanine D (17), were assigned as new natural products. In addition, compounds 10 and 11 represent the first natural occurrence of 7,8-didehydroprotoberberines. Compound 3, Pseudocolumbamine (12), palmatine (15), and pseudopalmatine (16) showed anti-AChE IC50 values of 8.4, 5.0, 0.4, and 1.8 muM, respectively.
Antiplasmodial alkaloids from Desmos rostrata.[Pubmed:19053513]
J Nat Prod. 2008 Dec;71(12):2057-9.
Two new alkaloids, desmorostratine (1) and discretine N-oxide (2), were isolated from the stem bark of Desmos rostrata, together with five known alkaloids, discretine (3), dehydrodiscretine (4), Pseudocolumbamine (5), predicentrine (6), and aristolactam AII (7). The structures were established on the basis of spectroscopic data, including mass spectrometry and 2D-NMR. Compound 1 was cytotoxic against KB cells (IC(50) 2.4 microM), while 2, 3, and 4 inhibited Plasmodium falciparum (IC(50) of 4.2, 1.6, and 0.9 microM, respectively).
Separation of protoberberine quaternary alkaloids from a crude extract of Enantia chlorantha by centrifugal partition chromatography.[Pubmed:15281263]
J Chromatogr A. 2004 Jul 2;1041(1-2):143-52.
High-performance centrifugal partition chromatography (HPCPC) has been successfully applied to the separation of four protoberberine quaternary alkaloids, namely palmatine, jatrorrhizine, columbamine and Pseudocolumbamine, from a methanolic extract (M1, 1.47 g) of Enantia chlorantha Oliver stem bark. For their isolation, two successive biphasic solvent systems composed of dichloromethane-methanol-water (48:16:36, v/v) were selected. The aqueous-rich phase was the stationary phase and the organic-rich phase was the mobile phase. The first system, containing potassium perchlorate, allowed to isolate 600 mg of palmatine, and to obtain 146 mg of a mixture (M2) containing only jatrorrhizine, columbamine and Pseudocolumbamine. The second biphasic system, prepared with water alkalinized with sodium hydroxide, was employed to isolate the M2 components. This system applied to the purification of 70 mg of M2 allowed to obtain 16 mg ofjatrorrhizine and 13 mg of columbamine. To obtain Pseudocolumbamine (16 mg), the elution mode was reversed, the aqueous-rich phase becoming the mobile phase, and the organic-rich phase becoming the stationary one. Analytical reversed-phase high-performance liquid chromatography, NMR, high-resolution mass spectrometry and UV spectrometry were used to verify the identity and the purity of the isolated compounds.
Alkaloids from Chasmanthera dependens.[Pubmed:17396979]
Planta Med. 1982 Dec;46(4):228-30.
A phytochemical investigation of the stem of Chasmanthera dependens Hochst led to the isolation and identification of five quaternary alkaloids: jatrorrhizine, columbamine, Pseudocolumbamine, magnoflorine and palmatine and nine tertiary non-phenolic alkaloids: tetrahydropalmatine, liriodenine, lysicamine, oxoglaucine, glaucine, anonaine, nornuciferine, norglaucine and O,O-dimethylcorytuberine.
Studies on Indonesian medicinal plants VI further alkaloids from Fibraurea chloroleuca.[Pubmed:17401818]
Planta Med. 1981 Jan;41(1):65-8.
From the stem and root bark of Fibraurea chloroleuca Miers the alkaloids magnoflorine, Pseudocolumbamine, dehydrocorydalmine and palmatrubine were isolated and identified by their spectral data. By means of TLC the alkaloids berberine and berberrubine were found to be present in minute amounts.
