DsbG, Recombinant ProteinCatalog# BCP26207 |
| Product Name | DsbG, Recombinant Protein | ||
| Full Product Name | DsbG, 18-248aa, E Coli, E Coli | ||
| Product Synonym Names | Thiol:disulfide interchange protein dsbG; ybdP; Thiol:disulfide interchange protein dsbG ECK0598; JW0597; Periplasmic disulfide isomerase/thiol disulphide oxidase | ||
| Product Gene Name | DsbG recombinant protein | ||
| Research Use Only | For Research Use Only. Not for use in diagnostic procedures. | ||
| Sequence | MEELPAPVKA IEKQGITIIK TFDAPGGMKG YLGKYQDMGV TIYLTPDGKH AISGYMYNEK GENLSNTLIE KEIYAPAGRE MWQRMEQSHW LLDGKKDAPV IVYVFADPFC PYCKQFWQQA RPWVDSGKVQ LRTLLVGVIK PESPATAAAI LASKDPAKTW QQYEASGGKL KLNVPANVST EQMKVLSDNE KLMDDLGANV TPAIYYMSKE NTLQQAVGLP DQKTLNIIMG NK | ||
| 3D Structure | ModBase 3D Structure for P77202 | ||
| Host | E Coli | ||
| Purity/Purification | > 95% by SDS-PAGE | ||
| Form/Format | Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 2 mM EDTA, 10% glycerol | ||
| Concentration | 1 mg/ml (determined by Bradford assay) (lot specific) | ||
| Antigen Species | E Coli | ||
| Preparation and Storage | Can be stored at 4 degree C short term (1-2 weeks).For long term storage, aliquot and store at -20 degree C or -70 degree C.Avoid repeated freezing and thawing cycles. | ||
| Other Notes | Small volumes of DsbG recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. | ||
| Related Product Information for DsbG recombinant protein | Dsb proteins control the formation and rearrangement of disulfide bonds during the folding of secreted and membrane proteins in bacteria. DsbG, a member of this family, has disulfide bond isomerase and chaperone activity. So DsbG was shown to interact with refolding intermediates of chemically denatured citrate synthase and prevents their aggregation in vitro. In addition to sharing sequence homology with the thiol disulfide exchange protein DsbC, DsbG likewise was shown to form a stable periplasmic dimer and it displays an equilibrium constant with glutathione comparable with DsbA and DsbC. DsbG was found to be expressed at approximately 25% the level of DsbC. Recombinant DsbG was expressed in E Coli and purified by conventional chromatography techniques. | ||
| Product Categories/Family for DsbG recombinant protein | Heat Shock Proteins | ||
| Applications Tested/Suitable for DsbG recombinant protein | SDS-PAGE | ||
| NCBI GI # | 90111151 | ||
| NCBI GeneID | 945224 | ||
| NCBI Accession # | NP_415137 | ||
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