Crystal VioletCAS# 548-62-9 |
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Quality Control & MSDS
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Chemical structure
3D structure
| Cas No. | 548-62-9 | SDF | Download SDF |
| PubChem ID | 11057 | Appearance | Powder |
| Formula | C25H30ClN3 | M.Wt | 407.98 |
| Type of Compound | N/A | Storage | Desiccate at -20°C |
| Synonyms | Basic Violet 3; Gentian Violet; Hexamethylpararosaniline chloride; Methyl Violet 10B | ||
| Solubility | DMSO : ≥ 100 mg/mL (245.11 mM) H2O : 5 mg/mL (12.26 mM; Need ultrasonic) *"≥" means soluble, but saturation unknown. | ||
| Chemical Name | [4-[bis[4-(dimethylamino)phenyl]methylidene]cyclohexa-2,5-dien-1-ylidene]-dimethylazanium;chloride | ||
| SMILES | [Cl-].CN(C)c1ccc(cc1)[C+](c2ccc(cc2)N(C)C)c3ccc(cc3)N(C)C | ||
| Standard InChIKey | ZXJXZNDDNMQXFV-UHFFFAOYSA-M | ||
| Standard InChI | InChI=1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1 | ||
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months. Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it. |
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| About Packaging | 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial. 2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial. 3. Try to avoid loss or contamination during the experiment. |
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| Shipping Condition | Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request. | ||
| Description | Crystal violet is a triarylmethane dye.
Target: Others
Crystal Violet is a component of Gram staining that allows one to recognize the difference between gram-positive and gram-negative bacteria with differential staining. The compound helps to access bacterial contamination of tissue culture samples. The staining response is due to the difference in the chemical and structural composition of the cell walls in different bacteria. Crystal Violet can also be used to test the different species of archaea and the cytostatic/cytotoxic effects on tumor cell lines [1-3]. References: | |||||
Crystal Violet Dilution Calculator
Crystal Violet Molarity Calculator
| 1 mg | 5 mg | 10 mg | 20 mg | 25 mg | |
| 1 mM | 2.4511 mL | 12.2555 mL | 24.511 mL | 49.022 mL | 61.2775 mL |
| 5 mM | 0.4902 mL | 2.4511 mL | 4.9022 mL | 9.8044 mL | 12.2555 mL |
| 10 mM | 0.2451 mL | 1.2256 mL | 2.4511 mL | 4.9022 mL | 6.1278 mL |
| 50 mM | 0.049 mL | 0.2451 mL | 0.4902 mL | 0.9804 mL | 1.2256 mL |
| 100 mM | 0.0245 mL | 0.1226 mL | 0.2451 mL | 0.4902 mL | 0.6128 mL |
| * Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations. | |||||
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Simultaneous spectrophotometric determination of crystal violet and malachite green in water samples using partial least squares regression and central composite design after preconcentration by dispersive solid-phase extraction.[Pubmed:28361487]
Environ Monit Assess. 2017 Apr;189(4):196.
In this paper, a simple, fast, and inexpensive method is introduced for the simultaneous spectrophotometric determination of Crystal Violet (CV) and malachite green (MG) contents in aquatic samples using partial least squares regression (PLS) as a multivariate calibration technique after preconcentration by graphene oxide (GO). The method was based on the sorption and desorption of analytes onto GO and direct determination by ultraviolet-visible spectrophotometric techniques. GO was synthesized according to Hummers method. To characterize the shape and structure of GO, FT-IR, SEM, and XRD were used. The effective factors on the extraction efficiency such as pH, extraction time, and the amount of adsorbent were optimized using central composite design. The optimum values of these factors were 6, 15 min, and 12 mg, respectively. The maximum capacity of GO for the adsorption of CV and MG was 63.17 and 77.02 mg g(-1), respectively. Preconcentration factors and extraction recoveries were obtained and were 19.6, 98% for CV and 20, 100% for MG, respectively. LOD and linear dynamic ranges for CV and MG were 0.009, 0.03-0.3, 0.015, and 0.05-0.5 (mug mL(-1)), respectively. The intra-day and inter-day relative standard deviations were 1.99 and 0.58 for CV and 1.69 and 3.13 for MG at the concentration level of 50 ng mL(-1), respectively. Finally, the proposed DSPE/PLS method was successfully applied for the simultaneous determination of the trace amount of CV and MG in the real water samples.
Adsorption Capability of Cationic Dyes (Methylene Blue and Crystal Violet) onto Poly-gamma-glutamic Acid.[Pubmed:28250349]
Chem Pharm Bull (Tokyo). 2017;65(3):268-275.
In this study, the adsorption capability of cationic dyes, which were methylene blue and Crystal Violet, by poly-gamma-glutamic acid (PGA) in a single or binary solution system was investigated. The effect of the molecular weight of PGA, initial dye concentration, solution pH, and temperature on the adsorption of dyes was evaluated. The adsorption mechanism of dyes onto PGA was the interaction between -COOH group on the PGA surface and the polarity groups of dyes. These results indicated that PGA is useful for removal of dyes and cationic organic compounds from a single or binary solution system.
Optimization of cellulose and sugarcane bagasse oxidation: Application for adsorptive removal of crystal violet and auramine-O from aqueous solution.[Pubmed:28160707]
J Colloid Interface Sci. 2017 May 15;494:223-241.
Cellulose (Cel) and sugarcane bagasse (SB) were oxidized with an H3PO4-NaNO2 mixture to obtain adsorbent materials with high contents of carboxylic groups. The oxidation reactions of Cel and SB were optimized using design of experiments (DOE) and response surface methodology (RSM). The optimized synthesis conditions yielded Cox and SBox with 4.8mmol/g and 4.5mmol/g of carboxylic acid groups, respectively. Cox and SBox were characterized by FTIR, TGA, PZC and solid-state (13)C NMR. The adsorption of the model cationic dyes Crystal Violet (CV) and auramine-O (AO) on Cox and SBox in aqueous solution was investigated as a function of the solution pH, the contact time and the initial dye concentration. The adsorption of CV and AO on Cox was described by the Elovich equation and the pseudo-first-order kinetic model respectively, while the adsorption of CV and AO on SBox was described by the pseudo-second-order kinetic model. Adsorption isotherms were well fitted by the Langmuir and Konda models, with maximum adsorption capacities (Qmax) of 1117.8mg/g of CV and 1223.3mg/g of AO on Cox and 1018.2mg/g of CV and 682.8mg/g of AO on SBox. Desorption efficiencies were in the range of 50-52% and re-adsorption capacities varied from 65 to 81%, showing the possibility of reuse of both adsorbent materials.
Multicenter evaluation of crystal violet decolorization assay (CVDA) for rapid detection of isoniazid and rifampicin resistance in Mycobacterium tuberculosis.[Pubmed:27982061]
Sci Rep. 2016 Dec 16;6:39050.
The aim of this multicenter study was to evaluate the performance of the Crystal Violet decolorization assay (CVDA) for detection of multidrug resistant tuberculosis (MDR-TB). This study was performed in 11 centers in two phases. A total of 156 isolates were tested for INH and RIF resistance. In the phase I, 106 clinical isolates were tested in the Center 1-7. In the phase 2, 156 clinical isolates were tested in the center 1-6, center 8-11. Eighty six of 156 tested isolates were the same in phase I. Agreements were 96.2-96.8% for INH and 98.1-98.7% for RIF in the phase I-II, respectively. Mean time to obtain the results in the phase I was 14.3 +/- 5.4 days. In the phase II, mean time to obtain the results was 11.6 +/- 3.5 days. Test results were obtained within 14days for 62.3% (66/106) of isolates in the phase I and 81.4% (127/156) of isolates in the phase II. In conclusion, CVDA is rapid, reliable, inexpensive, and easy to perform for rapid detection of MDR-TB isolates. In addition, it could be adapted for drug susceptibility testing with all drugs both in developed and developing countries.
