ADAMTS13, Recombinant ProteinCatalog# BCP05318 |
| Product Name | ADAMTS13, Recombinant Protein | ||
| Full Product Name | ADAMTS13, Recombinant, Human (A disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 13) | ||
| Product Gene Name | ADAMTS13 recombinant protein | ||
| Research Use Only | For Research Use Only. Not for use in diagnostic procedures. | ||
| Chromosome Location | Chromosome: 9; NC_000009.11 (136279459..136324525). Location: 9q34 | ||
| OMIM | 274150 | ||
| 3D Structure | ModBase 3D Structure for Q76LX8 | ||
| Purity/Purification | >90%, as determined by SDS-PAGE and visualized by silver stain. | ||
| Form/Format | Supplied as a liquid in 25mM HEPES, 300mM sodium chloride, pH 8.0 | ||
| Concentration | 0.33 mg/ml (lot specific) | ||
| N-terminal Sequence Analysis | Ala75 | ||
| Biological Activity | Measured by its ability to cleave the fluorogenic peptide substrate, FRETS-VWF73. The specific activity is >10pmol/min/ug. | ||
| Grade | Purified | ||
| Endotoxin | < 1 EU/ug (LAL) | ||
| Activity Assay Protocol | Materials:-Assay Buffer (TCNB): 50mM Tris, 2 mM CaCl2, 0.05% Brij35, pH 5.5-BCP636953: Recombinant human ADAMTS13 -Substrate: FRETS-VWF73, 100uM in DMSO-F16 Black Maxisorp Plate-Fluorescent Plate ReaderAssay:1. Dilute BCP636953 to 5ug/ml in assay buffer.2. Dilute substrate to 8uM in assay buffer.3. Load 50ul of dilute BCP636953 into a plate, and start the reaction by adding 50ul of 8uM substrate. Include a Substrate Blank containing 50ul of Assay Buffer and 50ul of 8ul substrate.4. Read at excitation and emission wavelengths of 340nm and 450 nm (top read), respectively, in kinetic mode for 5 minutes.5. Calculate specific activity:Sp Act (pmol/min/ug)=*Adjusted Vmax (RFU/min) x **Conversion Factor (pmol/RFU)/amount of enzyme (ug)*Adjusted for substrate blank**Derived using calibration standard FRETS25STD1Final Assay Conditions: rhADAMTS13: 0.25ug | ||
| Preparation and Storage | Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.Shipping: Dry Ice. | ||
| Other Notes | Small volumes of ADAMTS13 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. | ||
| Related Product Information for ADAMTS13 recombinant protein | ADAMTS13 (a disintegrin and metalloproteinase with thrombospondin motifs 13), also known as von Willebran Factor (vWF) cleaving protease, is a member of the family of secreted zinc proteases with a multi-domain structure (1-3). The protein precursors consist of a signal peptide and following domains: pro, catalytic, disintegrin-like, TS type 1 motif, cystein-rich, spacer, a second set of seven TSP1 repeats, and two CUB domins. The only known substrate of ADAMTS13 is vWF, a blood glycoprotein with two homeostatic functions (4). It is required for platelet adhesion to sites of vascular damage and acts as a carrier protein for blood-clotting factor VIII in the circulation. It exists in plasma as multimers, the largest of which effectively mediate platelet adhesion. ADAMTS13 cleaves multimeric vWF in the A2 domain at the position, Tyr 1605-Met 1606. Defect in ADAMTS13 activity is a cause of congenital thrombotic thrombocytopenic purpura (TTP), also known as Upshaw-Schulman syndrome. Lack of ADAMTS13 activity allows unusually large vWF (UlvWF) to occur in plasma (5). These UlvWF multimers have tendency to agglutinate circulating platelets at sites with high levels of shear stress to cause TTP.Recombinant protein corresponding to Gln34-Trp688 with a C-terminal 10X-His tag from human ADAMTS13 (Accession #NP_620594), expressed in CHO cell line. The purified rhADAMTS13 starts at the N-terminus of the pro domain and ends in the spacer domain. | ||
| Product Categories/Family for ADAMTS13 recombinant protein | Molecular Biology; MB-Matrix Metalloproteinases | ||
| NCBI GI # | 73695936 | ||
| NCBI GeneID | 11093 | ||
| NCBI Accession # | NP_620596.2 | ||
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